The chemical name of HEPES is N-(2-Hydroxyethyl)piperazine-N’-2-ethanesulfonic acid. HEPES is a Zwitterionic buffering agent used to maintain the pH of media used in cell cultures. It is one of Good's twelve buffers that has a pKa value similar to its pH value, making it an ideal buffer for pH maintenance.
HEPES is very useful in maintaining the structure and function of enzymes. HEPES is a free acid and is available in three different grades: Bio Industrial, Bio Synthesized and Bio Ultra. The HEPES free acid Bio Ultra grade is available as a US manufactured buffer that is tested and approved to ensure that it is in its purest form. Through our different product lines, HEPES free acid is also available in multiple sizes and packages to meet your specific needs.