Researchers at the RIKEN Omics Science Center (OSC) have adapted a new gene expression technique to accurately and quantitatively measure gene expression levels using only 100 nanograms of total RNA.
The technique, which pairs RIKEN’s Cap Analysis of Gene Expression (CAGE) protocol with the Helicos Genetic Analysis System developed by Helicos BioSciences, opens the door to the detailed analysis of gene expression networks and rare cell populations.
The Cap Analysis of Gene Expression (CAGE) protocol enables not only high-throughput gene expression profiling, but also simultaneous identification of transcriptional start sites (TSS) specific to each tissue, cell or condition.
With HeliScopeCAGE, the OSC research team has adapted the existing CAGE protocol for use with the revolutionary HeliScope Single Molecule Sequencer.
Unlike earlier sequencers the HeliScope Sequencer actually sequences the DNA strand itself, enabling direct, high-precision measurement.